Imam signed the photo for Seyyed Ali Khamenei , who had been a prominent pupil of Imam and his right-hand throughout the struggle for the Islamic Revolution.
The memorial photo has been mounted on a wall by the supreme leader. The historic picture is also now widely been circulating on social networking website such as Telegram.
The developments come as millions of Iranians are marking the anniversary of the eternal departure of Imam Khomeini, 28 years ago.
The anniversary of his passing falls on Sunday this year.
Leader of the Islamic Revolution Ayatollah Seyyed Ali Khamenei will address the gathering of mourners at the holy shrine of Imam Khomeini.